Journal: Cell reports
Article Title: High-resolution phenotyping of early acute rejection reveals a conserved alloimmune signature.
doi: 10.1016/j.celrep.2021.108806
Figure Lengend Snippet: Figure 5. Ly6Chi/CD62L+ inflammatory monocytes are high frequency in acute rejection at day 5 (A) UMAP of myeloid cells (CD3/CD19/NK1.1), visualizing 12 clusters across all 15 samples. (B) Heatmap of median expression for the 12 clusters identified by FlowSOM shown in (A).
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Gr1 (RB6-8C5); Clone preferentially binds Ly6G Fluidigm Cat#3141005B; Lot#1201813 CD11c (N418) Fluidigm Cat#3142003B; Lot#1201819 GITR (DTA1) Fluidigm Cat#3143019B; Lot#2171502 MHCI (28-14-8) Fluidigm Cat#3144016B; Lot#0151802 CD69 (H1.2F3) Fluidigm Cat#3145005B; Lot#3511606 CD5 (53-7.3) Fluidigm Cat#3146012B; Lot#2041519 CD45 (30-F11) Fluidigm Cat#3147003B; Lot#3181713 CD11b (M1/70); Alt. name: Mac-1 Fluidigm Cat#3148003B; Lot#1621803 CD19 (6D5) Fluidigm Cat#3149002B; Lot#0581828 CD24 (M1/69) Fluidigm Cat#3150009B; Lot#0901508 CD25 (3C7) Fluidigm Cat#3151007B; Lot#3511605 CD3ε (145 2X11) Fluidigm Cat#3152004B; Lot#2601606 CD8a (53 6.7) Fluidigm Cat#3153012B; Lot#0101806 CTLA4 (UC10-4B9); Alt. name: CD152 Fluidigm Cat#3154008B; Lot#1841809 TCRgd (UC7-13D5) Biolegend Cat#107502 CCR6 (29-2L17); Alt. name: CD196 Fluidigm Cat#3156016A; Lot#0581825 CD80 (16-10A1); Alt. name: B7-1 Biolegend Cat#104702 FOXP3 (FJK-16 s) Fluidigm Cat#3158003A; Lot#0691401 F4/80 (BM8) Fluidigm Cat#3159009B; Lot#0541707 CD62L (MEL-14) Fluidigm Cat#3160008B; Lot#2631805 Ly6C (HK1.4) Fluidigm Cat#3162014B; Lot#2341706 CCR7 (4B12); Alt. name: CD197 Fluidigm Cat#3164013A; Lot#0901709 NK1.1 (PK136) Fluidigm Cat#3165018B; Lot#0101808 CD27 (LG.3A10) Biolegend Cat#124202 NKp46 (29A1.4); Alt. name: CD335 Fluidigm Cat#3167008B; Lot#1501808 CD21 (7G6) Fluidigm Cat#3168010B; Lot#2371409 TCRb (H57 597) Fluidigm Cat#3169002B; Lot#0761712 CD40L (MR1); Alt. name: CD154 Fluidigm Cat#3170011B; Lot#2041508 CD44 (IM7) Fluidigm Cat#3171003B; Lot#3421608 CD4 (RM4-5) Fluidigm Cat#3172003B; Lot#1201808 Granzyme B (GB11) Fluidigm Cat#3173006B; Lot#1511802 LAG3 (C9B7W) Fluidigm Cat#3174019B; Lot#1201814 CD127 (A7R34); Alt. name: IL7Ra Fluidigm Cat#3175006B; Lot#0101807 B220 (RA3-6B2); Alt. name: CD45R Fluidigm Cat#3176002B; Lot#0321704 MHCII I-A/I-E (M5/114.15.2); Binds both donor and recipient MHC Fluidigm Cat#3209006B; Lot#2851707 Fc Block: TruStain FcX (anti-mouse CD16/32) Antibody Biolegend Cat#101320 Critical commercial assays Dead cell removal kit StemCell Cat#17899 40 micron nylon mesh Fisher Cat#352340 Cisplatin Fluidigm Cat#201195 Cell staining buffer Fluidigm Cat#201068 Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat#201060 (Continued on next page) e1 Cell Reports 34, 108806, March 2, 2021
Techniques: Expressing
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